Please use this identifier to cite or link to this item: https://hdl.handle.net/10419/243818 
Year of Publication: 
2020
Citation: 
[Journal:] Energy Reports [ISSN:] 2352-4847 [Volume:] 6 [Issue:] 1 [Publisher:] Elsevier [Place:] Amsterdam [Year:] 2020 [Pages:] 751-756
Publisher: 
Elsevier, Amsterdam
Abstract: 
This work aimed to: (i) assess the performance of Novozyme® 435 for FAME production from waste cooking oil (WCO) under different oil acid values and enzyme-to-oil ratios; (ii) test different regeneration methods and assess the regenerated enzyme's performance; and (iii) determine FAME yield in continuous operation regime. Oil acid values up to 104 mg KOH.g-1 and enzyme-to-WCO ratios of up to 20:80 (% wt.) were studied, with maximum yield recorded for increasing acid values and enzyme-to-WCO ratio. FAME production from WCO yield ranging 50%-80% was recorded for 5 consecutive production cycles (8h each, in batch regime) with no regeneration of the enzyme. Among the enzyme regeneration methods tested, the highest FAME yield was recorded using t-butanol washing followed by enzyme incubation in the WCO before reusing it. Operation in continuous regime, including enzyme regeneration, produced the highest FAME yield (maximum of 86 %). Thus, effectiveness of the regenerating process and efficiency of continuous production of FAME from WCO (up to 576h) were observed.
Subjects: 
Biodiesel
FAME
Waste cooking oil
Enzyme regeneration
Novozyme® 435
Persistent Identifier of the first edition: 
Creative Commons License: 
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Document Type: 
Article
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